Detect and analyze periodic oscillation of the fluorescene time-series signal.
FluorescenceOscillation.Rmd is the R markdown file that read the data files, conduct time-series analysis and display statistical plots.
ROI-ZXY.ijm is the ImageJ script that extract pixel-wise time-series data. It contains four macros.
Cell-ROI is the macro that outline the cellular region based on the z-stacks summation image (accumulated flurorescence).
ZXY-Cell-Crop is the macro that extract the dataset for this image window. Each column of the Results Table contains a time-series datasets of the corresponding pixel. Row one contains the pixel coordinate, row two contains a indicator that tell whether this pixel is in the outlined ROI (1:Perpheral; 2:Cell).
ZXY-BG-Crop is the macro that extract the dataset for this image window but without selection (whole crop window). This is used for extracting background dataset which is labeled with a indicator (0:BG).
ZXY-BG-Rec is the macro that extract the dataset whithin this image window with a rectangle selection. This is used for extracting background dataset which is labeled with a indicator (0:BG).
TimeStamp is the macro that extract time stamp, time interval between each frame (1/frame rate) and exposure time from the imaging stacks.